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Sericulture Research & Development Council

Allied Sectors Occupations

Silkworm Seed/Egg production

Disinfection of grainage and implements

  • Bamboo made house with thatched roof and mud-plastered wall is preferable for grainage.
  • Disinfect the grainage rooms and appliances prior to commencement of operation.
  • Wash the rooms and appliances with 5% bleaching powder solution before one day.
  • Dry the appliances in sun shine for 3-4 hours before one day.
  • Disinfect the rooms and appliances with a mixture of 2% formalin, 0.5-1% lime and 0.5% detergent solutions @ 1L/ sq.m after one day.

Pupal examination

  • Separate out the melted, flimsy and good cocoons.
  • Take out the gut portion and observe under microscope.
  • Reject the lot in case there is incidence of pebrine.
  • Disinfect the contaminated room before the arrival of new lots.

Preservation and protection of seed cocoons

  • Immediately after the receipt of seed cocoons, spread them on trays in a single layer to facilitate good aeration.
  • Reject the seed cocoons which are melted, uzi infested, flimsy and not conforming to the characteristics of parent races.
  • Preserve only the healthy seed cocoons in trays for further processing.
  • Provide cross ventilation in the preservation room.
  • Maintain temperature at 25 ± 1˚C, relative humidity of 75 ± 5%, 16 hour light and 8 hour dark conditions in the cocoon preservation room.
  • Maintain complete darkness one day prior to emergence to avoid irregular emergence of moths.

Early eclosion/ artificial eclosion/ forced eclosion of moths

  • This helps in determining the disease freeness of a batch and helps in minimizing the loss to grainage.
  • For early emergence of moths, 50-60 seed cocoons are taken from individual lots and placed into an artificial eclosion box.
  • Maintain the temperature at 32-33 0C with the help of thermostat. This accelerates the development of pupae and moth emerges early.
  • Examine the early emerged female moths of respective lots are under microscope to know the disease freeness of lots.

Synchronization of emergence of moths, pairing, depairing and oviposition

  • Keep the cocoon preservatio0n room dark before the expected day of emergence of moths.
  • In case of variation in development of male or female pupae, the development of male pupae can be arrested by preserving them at 5-7 0C and 75 + 5% relative humidity for 3-4 days.
  • Only healthy and active moths are taken for pairing.
  • After 1-2 hours of emergence, the male and female moths of respective combination are allowed for 3.5 to 4 hours of pairing.
  • At the time of depairing, the male and female moths are to be moved side way so that the moths are separated easily without causing injury to reproductive organs.
  • The mated female moths are taken in a separate container and induced for urination.
  • Moths are placed on egg sheet and covered with cellules and kept in semi dark condition for oviposition.
  • Under proper preservation (5-7 0C), male moths can be used for second pairing by giving 1-2 hours rest.
  • Throughout the process of pairing, depairing and oviposition, optimum temperature of 25 ± 1 0C and relative humidity of 75 ± 5% should be maintained.

Moth examination

  • Examine the mother moth either individually or in mass for pebrine disease.

Surface sterilization of eggs

  • Dip the egg sheets in 2% formalin for 10-15 minutes. This helps in removal of pathogens adhering to the egg shell and further prevents secondary contamination.
  • Wash the eggs in formalin solution. It helps in firm adherence of eggs to the sheet.

Incubation eggs

  • Maintain temperature of 25 ± 1˚C, relative humidity of 75 ± 5%, 16 hours of light and 8 hours darkness.
  • During the pinhead stage or before two days of hatching, maintain total darkness for uniform development of embryo and hatching of larvae at a time on a single day.

Source

  • Directory of Sericulture Technology 2008, Karnataka State Sericulture Research and Development Institute, Bangalore- 5 60 062.